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PHL Murine Immunohistochemistry Database
Notes on Techniques, Abbreviations, and Acronyms:
The following key to abbreviations used in the list assumes some basic knowledge
of immunohistochemistry.
Mouse monoclonal antibodies on mouse tissues: Use of DAKO's ARK or Vector's MOM kits
can reduce background staining when mouse monoclonals are used on mouse tissues. There may
be other kits and other methods available that are not listed here.
Many of the techniques may be affected by the type or duration of the fixation used.
Please contact us with questions regarding fixation or to request additional information.
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Abbreviations |
| General |
| 4C |
4° celcius |
| 60’ |
60 minutes |
| AR Citra |
Antigen Retrieval Citra (citrate buffer) by BioGenex |
| dH2O |
distilled water |
| EDTA |
Ethylenediaminetetracetic acid |
| HR. |
Hour |
| MW |
microwave |
| no MW |
no microwave |
| no Tr. |
no Trypsin |
| OCT |
Fresh frozen cryostat sections |
| ON |
overnight |
| PC |
pressure cooker |
| RT |
room temperature |
| Tr. |
Trypsin |
| |
| Chromagen/Substrate |
| AP |
Alkaline Phosphatase |
| DAB |
3,3’-Diaminobenzidine Tablets by Sigma, Liquid by DAKO |
| DAPI |
DAPI by VECTOR |
| HRP |
Horseradish Peroxidase |
| Nova Red |
Nova Red Substrate Kit, SK-4800 by VECTOR |
| Texas Red |
Texas Red Avidin D by VECTOR |
| VECTOR Blue |
Alkaline Phosphatase Substrate Kit III, SK-5300, by VECTOR |
| VECTOR Red (AP Red) |
Alkaline Phosphatase Substrate Kit I, SK-5100, by VECTOR |
| |
| Kits |
| ABC only |
Avidin – Biotinylated Enzyme Complex Only; used when biotinylated primary antibodies are applied to tissue, VECTOR |
| ApopTag |
Apoptosis Detection Kit, Serologicals Corp.
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| ARK |
Animal Research Kit, DAKO |
| Fluorescein 2Ab |
Fluorescein a/Rabbit IgG (H+L), affinity purified, made in goat, VECTOR |
| Goat e. |
Goat Elite Kit, VECTOR |
| Guinea Pig |
Guinea Pig Kit, VECTOR |
| Human e. |
Human Elite Kit, VECTOR |
| LSAB+ |
LSAB+ (labelled streptavidin biotin) Kit, DAKO |
| M.O.M. |
Mouse primary antibody On Mouse Tissue Kit, VECTOR |
| Mouse e. |
Mouse Elite Kit, VECTOR |
| Mouse e./F(ab’)2/m |
Blocking serum and Avidin-Biotinylated complex from Mouse Elite Kit, VECTOR; secondary antibody is biotinylated a/mouse Immunoglobulins (Biotin Rabbit F(ab’)2), DAKO |
| NDS |
Normal Donkey Serum |
| NGS |
Normal Goat Serum |
| NHS |
Normal Horse Serum |
| NRS |
Normal Rabbit Serum |
| Rabbit e. |
Rabbit Elite Kit, VECTOR |
| Rat e. |
Rat Elite Kit, Vector |
| Rat e./2Ab mouse adsorbed |
Blocking serum and Avidin-Biotinylated complex from Rat Elite Kit, VECTOR; secondary antibody is biotinylated anti-Rat IgG (H+L) made in rabbit, mouse adsorbed, VECTOR |
| Sheep e. |
Sheep Elite Kit, VECTOR |
| TSA |
Tyramide Signal Amplification, PerkinElmer Life Science, Inc. |
Antibody Manufacturers |
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The list of manufacturers refers only to those cited in this database. It is not intended
to be comprehensive.
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| Lifespan Biosciences |
Lifespan
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| AbD Serotec |
www.ab-direct.com
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| ABGENT |
www.abgent.com
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| Affinity BioReagents, Inc. |
www.bioreagents.com
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| Alexis Biochemicals |
www.alexis-e.biz
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| Aviva Systems Biology |
www.avivasysbio.com
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| BD Biosciences |
www.bdbiosciences.com
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| Biogenesis, Inc. |
www.biogenesis.co.uk
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| BioGenex |
www.biogenex.com
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| BIOMOL Research Laboratories, Inc. |
www.biomol.com
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| BioSource International, Inc. |
biosource.com
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| CALBIOCHEM |
www.calbiochem.com
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| CALTAG Laboratories |
www.caltag.com
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| Capralogics, Inc. |
www.capralogics.com
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| Cayman Chemical |
www.caymanchem.com
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| CEDARLANE Laboratories Limited |
www.cedarlanelabs.com
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| Cell Signaling Technology, Inc. |
www.cellsignal.com
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| CHEMICON International, Inc. |
www.chemicon.com
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| Cortex Biochem, Inc. |
www.cortex-biochem.com
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| Covance Research Products, Inc. |
www.CRPinc.com
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| DAKO Corporation |
www.dakousa.com
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| Endogen |
www.endogen.com
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| Exalpha Biologicals, Inc. |
www.exalpha.com
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| GeneTex, Inc. |
www.genetex.com
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| Gentest, see BD Biosciences |
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| GenWay Biotech, Inc. |
www.genwaybio.com
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| ICN Biomedicals, Inc., see MP Biomedicals |
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| ICN/Cappel Biomedicals, Inc., see MP Biomedicals |
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| Immunology Consultant Laboratory, Inc. |
www.icllab.com
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| ImmunoStar Incorporated |
www.immunostar.com
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| Invitrogen Life Technologies |
www.invitrogen.com
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| JICA (Japan Institute For The Control of Aging) |
www.fsinet.or.jp/~jica
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| KAMIYA Biomedical Company |
www.kamiyabiomedical.com
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| Lifespan Biosciences |
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| Molecular Probes, Inc. |
www.probes.com
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| MP Biomedicals |
www.mpbio.com
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| NeoMarkers, Inc. |
www.labvision.com
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| Noncommercial |
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| Novocastra Laboratories Ltd. |
www.novocastra.co.uk
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| Novus Biologicals, Inc. |
www.novus-biologicals.com
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| Oncogene Research Products |
www.apoptosis.com
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| PharMingen, see BD Biosciences |
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| Promega Corporation |
www.promega.com
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| ProteinTech Group, Inc. |
www.ptglab.com
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| R&D Systems, Inc. |
www.rndsystems.com
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| Research Diagnostics, Inc. |
www.researchd.com
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| Roche Applied Science Roche Diagnostic Corporation |
www.roche-applied-science.com
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| Rockland |
www.rockland-inc.com
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| Santa Cruz Biotechnology, Inc. |
www.scbt.com
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| Serotec |
www.serotec.co.uk
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| Sigma |
www.sigma-aldrich.com
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| Signet Laboratories, Inc. |
www.signetlabs.com
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| Southern Biotechnology Associates, Inc. |
www.southernbiotech.com
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| The Binding Site |
www.bindingsite.com
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| Torry Pines Biolabs, Inc. |
www.chemokine.com
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| Transduction Laboratories, see BD Biosciences |
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| Upstate |
www.upstatebiotech.com
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| US Biological |
www.usbio.net
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| VECTOR Laboratories, Inc. |
www.vectorlabs.com
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| Ventana |
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| Zymed Laboratories, Inc. |
www.zymed.com
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