(HUMAN rDNA)    88/646

AMPOULE CONTENTS

Each ampoule contains the residue after freeze-drying of 1 ml of a solution containing: approximately 1 microgram GM-CSF; 0.9% sodium chloride; 0.1% trehalose; 0.1% human serum albumin. The ampoules do not contain bacteriostat.

UNITAGE

The assigned potency is 10,000 international units (IU) per ampoule.

CAUTION

The preparation contains material of human origin which has been tested and found negative for HB_sAg and HIV antibody. However, as with all materials of biological origin, this preparation should be regarded as potentially hazardous to health. It should be used and discarded according to your own laboratory's safety procedures. Such safety procedures probably will include the wearing of protective gloves and avoiding the generation of aerosols. Care should be exercised in opening ampoules or vials to avoid cuts. This preparation is not for administration to humans.

USE OF AMPOULED MATERIAL

For all practical purposes, each ampoule contains the same quantity of the substances listed above. Dissolve the total contents of the ampoule with 0.5 ml of sterile distilled water. Rinse the ampoule with 0.4 ml of sterile phosphate buffered saline (PBS) and make up the total volume to 1 ml with PBS. This solution should now be at a concentration of 10,000 international units/ml. Use carrier protein where extensive dilution is required. No attempt should be made to weigh out any portion of the freeze-dried material. Unopened ampoules should be stored at -20^oC. For economy of use, it is recommended that the solution be sub-divided into several small aliquots and stored at -40^oC or below.

CITATION

In all publication in which this preparation is used as any assay calibrant, it is important that the title of the preparation and ampoule code be cited correctly.

This reagent is available in small amounts (approximately 1 microgram/sample) for use in the calibration of in vitro bioassays and in-house standards only and are not to be used for experimental purposes. Reference reagents are available to investigators with peer-reviewed support, at institutions or at commercial establishments and are limited to one sample of each reagent per year.