INTERNATIONAL STANDARD FOR EPIDERMAL GROWTH FACTOR (EGF)

AMPOULE CONTENTS

Each ampoule contains the residue after freeze-drying of 0.5 ml of a solution that contained: 4 microgram/ml EGF; 10 mg/ml trehalose; 10 mmol/l sodium phosphate, pH 6.5.

UNITAGE

The assigned potency is 2000 International Units (IU) per ampoule.

CAUTION

All materials of biological origin should be regarded as potentially hazardous to health. This preparation is not for administration to humans.

 

USE OF AMPOULED MATERIAL

The standard is intended for calibration of local standards. For all practical purposes, each ampoule contains the same quantity of EGF. The entire contents of each ampoule should be completely dissolved in a known volume of suitable solvent. It is recommended that, were possible, buffer containing carrier protein should be used.

STORAGE

This material should be used and discarded according to your own laboratory safety procedures.

CITATION

In all publications in which this preparation is used as an assay calibrant, it is important that the title of the preparation and ampoule code be cited correctly.

This reagent is available in small amounts (approximately 4 microgram/sample) for use in the calibration of in vitro bioassays and in-house standards only and is not to be used for experimental purposes. Reference reagents are available to investigators with peer-reviewed support, at institutions or at commercial establishments and are limited to one sample of each reagent per year.